清道夫受体在树突状细胞提呈口蹄疫病毒VP1-VP4抗原中的作用

doi:10.11843/j.issn.0366-6964.2014.01.018

Abstract

Abstract:

The aim of this study was to investigate the role of the scavenger receptors on dendritic cells in presentation of recombinant VP1-VP4 of foot-and-mouth disease virus (FMDV) to T cells in vitro. The prokaryotic expression vector of pET-32a-VP1-VP4 was constructed and the VP1-VP4 protein was expressed and purified. Bone marrow-derived dendritic cells (BMDCs) pretreated with scavenger receptor inhibitors were pulsed with recombinant VP1-VP4 antigen and then cocultured with lymph node T cells. The culture supernatants were harvested at different time points for
determining IFN-γ contents with ELISA. The results showed that the IFN-γ levels of experimental groups were significantly higher than that of control group at different time points (P＜0.01). Our data indicate that scavenger receptors on BMDCs are able to recognize recombinant VP1-VP4 protein and play a negative regulatory role in antigen presentation of BMDCs to initiate lymph node T cell responses.

CLC Number:

">S852.659.6

GAO Yun-huan, LI Na, DONG Chang-hai, TANG Ran-xiao, LI Li-min, WANG Jia-xin. The Role of Scavenger Receptors on Dendritic Cells in Presenting VP1-VP4 Antigen of Foot-and-Mouth Disease Virus to T Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 129-135.

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The Role of Scavenger Receptors on Dendritic Cells in Presenting VP1-VP4 Antigen of Foot-and-Mouth Disease Virus to T Cells

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